1.	Splinkerette PCR - Methods
	[Splinkerette PCR Methods - Digestion to remove genomic DNA fragments with splinkerette adaptor ligated on both ends]
	
	Splinkerette adaptor ligation : 10x NEB buffer : EcoRV(20 U/microL) : Acetylated BSA (10 mg/mL) : Double distilled Water
	Mixture: sterile_microfuge_tube2.contents, neb3, ecorv, bsa, water
	
	N Target Ratio	L
	5 40:10:1:1:48	100
	
	Citation:
	#################################
	@Article{ NatProtSplinkerettePCR2009,
		TITLE = "{A high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites}",
		AUTHOR = "Anthony G Uren and Harald Mikkers and Jaap Kool and Louise van der Weyden and Anders H Lund and Catherine H Wilson and Richard Rance and Jos Jonkers and Maarten van Lohuizen and Anton Berns and David J Adams",
		JOURNAL = "Nature Protocols",
		YEAR = "2009",
		VOLUME = "4",
		NUMBER = "5",
		PAGES = "789--798"
	}
	#################################
	
2.  Touchdown PCR

	Mixture: pcr_buffer, mgcl2, template_dna, dntp, f_primer, r_primer, dna_pol, ddw
	Target Ratio: 5 : 3 : 10 : 5 : 1 : 1 : 0.25 : 24.75 => 500 : 300 : 1000 : 500 : 100 : 100 : 25 : 2475

	N Target Ratio	 L
	8 20:12:40:20:4:4:1:99	200
	
	Citation:
	#################################
	@Misc{ webOpenWetWare,
		author = "{OpenWetWare}",
		month = "",
		year = "2009",
		note = "http://openwetware.org/wiki/Main\_Page",
		annote = ""
	}
	#################################

3.	Methods in Molecular barcodes paper
	[Methods in Molecular barcodes - PCR of purified products]

	Mixture: rxn_buffer, datp, dgtp, dttp, dctp, f_primer, r_primer, rxn_tube3.contents, taq, sdw
	Target Ratio: 1 : 0.2 : 0.2 : 0.2 : 0.2 : 0.5 : 0.5 : 1 : 0.04 : 6.16 =>  100 : 20 : 20 : 20 : 20 : 50 : 50 : 100 : 4 : 616

	N Target Ratio	L
	10 50:10:10:10:10:25:25:50:2:313	505

	Citations:
	#################################
	@Book{ bookDNAbarcodes2012,
		title = "{DNA Barcodes: Methods and Protocols}",
		author = "Ida Lopez and David L. Erickson",
		publisher = "Humana Press",
		year = "2012",
		annote = ""
	}
	@Misc{ webOpenWetWare,
		author = "{OpenWetWare}",
		month = "",
		year = "2009",
		note = "http://openwetware.org/wiki/Main\_Page",
		annote = ""
	}
	#################################

4.	Silver - Restriction Digest

	Mixture: 700 ng BioBrick vector (BBa_V0002 or Bba_V0100) - 7 L, 1 L 10 x BSA, 1 L 10x buffer, 0.2 L enzyme 1 (20 units/L), 0.2 L enzyme 2 (20 units/L), 0.6 L distilled water = total 10 L
	Target Ratio: 7 : 1 : 1 : 0.2 : 0.2 : 0.6 => 70 : 10 : 10 : 2 : 2 : 6

	N Target Ratio	L
	6 35:5:5:1:1:3	50

	Citation:
	#################################
	@Misc{ webOpenWetWare,
		author = "{OpenWetWare}",
		month = "",
		year = "2009",
		note = "http://openwetware.org/wiki/Main\_Page",
		annote = ""
	}
	#################################
	
5.	Blackburn - Yeast Colony PCR
	Mixture: 2uL 5X Q-solution, 1uL 10X PCR Buffer, 0.2uL dNTPs (10mM each), 0.2uL foward primer (100uM), 0.2uL reverse primer (100uM), 0.1uL Taq, 5.3uL ddH2O
	Target Ratio: 2 : 1 : 0.2 : 0.2 : 0.2 : 0.1 : 5.3 =>  20 : 10 : 2 : 2 : 2 : 1 : 53

	N Target Ratio	L
	7 20:10:2:2:2:1:53	90
	
	Citation:
	#################################
	@Misc{ webOpenWetWare,
		author = "{OpenWetWare}",
		month = "",
		year = "2009",
		note = "http://openwetware.org/wiki/Main\_Page",
		annote = ""
	}
	#################################
	
	#################################
	@Article{ 2009_Microsoft_BioCoder,
		AUTHOR = "{BioCoder: A Programming Language for Biology Protocols, Microsoft Research India, http://research.microsoft.com/en-us/um/india/projects/biocoder/}",
		TITLE = "",
		YEAR = 2009,
		MONTH = "December",
		ANNOTE = ""
	}
	#################################